User:Wayne Decatur/Haloarcula Large Ribosomal Subunit With Azithromycin

Azithromycin is a 15-membered macrolide antibiotic of the azalide subclass. The structure of this clinically important antibiotic has been solved bound to the Haloarcula marismortui large ribosomal subunit revealing its mode of inhibiting protein synthesis.

The basic details of the Haloarcula marismortui large ribosomal subunit are described in its own entry while the structure of azithromycin (zithromax) and its interaction with the ribosome are shown below on this page.

Mechanism of action
Azithromycin sits in the tunnel where it would occlude the nascent polypeptide chain from transiting through the subunit.

Summary: Azithromycin, like other macrolides, binds in the polypeptide exit tunnel near its start the polypeptide exit tunnel inhibiting protein synthesis by blocking the progression of nascent polypeptides through the tunnel. The polypeptide exit tunnel is described here.

About this Structure
1m1k is a 30 chains structure of sequences from Haloarcula marismortui. Full crystallographic information is available from OCA. A version of 1m1k optimized for use online as described here is used on this page. You may wish to examine the basics of the subunit.

Related Structures

 * Haloarcula Large Ribosomal Subunit
 * Interactions between Antibiotics and the Ribosome
 * 1nwy The Large Ribosomal Subunit From Deinococcus radiodurans complexed with Azithromycin
 * 1yhq Haloarcula marismortui large ribosomal subunit containing the mutation G2099A (A2058 in E. coli) complexed with Azithromycin - despite the change to the eubacterial-like adenosine at this position azithromycin binds essentially the same way to either Haloarcula subunit
 * 1kd1 The Large Ribosomal Subunit From H. marismortui complexed with the 16-membered macrolide spiramycin
 * 1k9m The Large Ribosomal Subunit From H. marismortui complexed with the 16-membered macrolide Tylosin
 * 1k8a The Large Ribosomal Subunit From H. marismortui complexed with the 16-membered macrolide Carbomycin A
 * 3cc2 The refined structure of the Large Ribosomal Subunit From H. marismortui

Additional Literature and Resources

 * Azithromycin at the DrugBank database
 * Azithromycin (zithromax) at Drugs.com
 * Azithromycin at Wikipedia